Anti-PTPRC Rat Antibody (PE (Phycoerythrin)-Cy7®) [clone: RA3-6B2]

Supplier: Tonbo Biosciences

C57Bl/6 splenocytes were stained with 0.125 ug PE-Cy7 Anti-Hu/Mo CD45R (B220) (60-0452) (solid line) or 0.125 ug PE-Cy7 Rat IgG2a isotype control (dashed line).

C57Bl/6 splenocytes were stained with 0.125 ug PE-Cy7 Anti-Hu/Mo CD45R (B220) (60-0452) (solid line) or 0.125 ug PE-Cy7 Rat IgG2a isotype control (dashed line).

60-0452-U025 60-0452-U100

10051-496EA 85.32 CAD

10051-496 10051-498

Anti-PTPRC Rat Antibody (PE (Phycoerythrin)-Cy7®) [clone: RA3-6B2]

Antibodies

The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB and CD45RO.

The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.

Type: Primary
Antigen: PTPRC
Clonality:
Clone: RA3-6B2
Conjugation: PE (Phycoerythrin)-Cy7®
Epitope:
Host: Rat
Isotype: IgG2a
Reactivity: Human, Mouse, Cat

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