Anti-CD4 Mouse Monoclonal Antibody (PE (Phycoerythrin)) [clone: RPA-T4]

Supplier: Tonbo Biosciences

Human peripheral blood lymphocytes were stained with 5 uL (0.5 ug) Anti-Human CD4 PE (50-0049) (solid line) or 0.5 ug Mouse IgG1 PE isotype control.

Human peripheral blood lymphocytes were stained with 5 uL (0.5 ug) Anti-Human CD4 PE (50-0049) (solid line) or 0.5 ug Mouse IgG1 PE isotype control.

50-0049-T025 50-0049-T100 50-0049-T500

10051-174EA 45.82 CAD

10051-174 10051-176 10051-178

Anti-CD4 Mouse Monoclonal Antibody (PE (Phycoerythrin)) [clone: RPA-T4]

Antibodies

The RPA-T4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

The RPA-T4 antibody is widely used as a phenotypic marker for human CD4 expression, and is cross-reactive with Chimpanzee CD4. This antibody recognizes a different epitope, and thus does not block binding, of the alternative Anti-Human CD4 antibody clone OKT4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065).

Type: Primary
Antigen: CD4
Clonality: Monoclonal
Clone: RPA-T4
Conjugation: PE (Phycoerythrin)
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity:

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