Anti-CD4 Rat Monoclonal Antibody (PerCP (Peridinin-Chlorophyll Protein Complex)-Cy5.5®) [clone: RM4-5]

Supplier: Tonbo Biosciences

C57Bl/6 splenocytes were stained with 0.25 ug PerCP-Cy5.5 Anti-Mouse CD4 (65-0042) (solid line) or 0.25 ug PerCP-Cy5.5 Rat IgG2a isotype control (dashed line).

C57Bl/6 splenocytes were stained with 0.25 ug PerCP-Cy5.5 Anti-Mouse CD4 (65-0042) (solid line) or 0.25 ug PerCP-Cy5.5 Rat IgG2a isotype control (dashed line).

65-0042-U025 65-0042-U100

10051-548EA 93.22 CAD

10051-548 10051-550

Anti-CD4 Rat Monoclonal Antibody (PerCP (Peridinin-Chlorophyll Protein Complex)-Cy5.5®) [clone: RM4-5]

Antibodies

The RM4-5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta2-domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will “compete” for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.

Type: Primary
Antigen: CD4
Clonality: Monoclonal
Clone: RM4-5
Conjugation: PerCP (Peridinin-Chlorophyll Protein Complex)-Cy5.5®
Epitope:
Host: Rat
Isotype: IgG2a
Reactivity:

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