AccuStart II PCR ToughMix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues.
- ToughMix formulation neutralize PCR inhibitors to ensure reliable assay performance with a variety of starting materials
- Stabilized 2X PCR SuperMix enables convenient room-temperature setup and is unaffected by repetitive freeze-thaw
- Supports reliable duplex assay performance
- Includes electrophoretic mobility dye
A key component of AccuStart PCR ToughMix is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. These antibodies bind the polymerase and keep it inactive prior to the initial PCR denaturation step. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Similar to Taq DNA polymerase, the activated polymerase in AccuStart II PCR ToughMix possesses 5’ ̶˃3’ DNA polymerase activity and a double-strand specific 5’ ̶˃3’ exonuclease. The polymerase does not have 3’-exonuclease activity and is free of any contaminating endo or exonuclease activities. PCR products generally contain non-templated dA additions and can be cloned using vectors that have a single 3'-overhanging thymine residue on each end.
GelTrack® Loading Dye is a mixture of blue and yellow electrophoresis-tracking dyes that migrate at approximately 4 kb and 50 bp. This optional component simplifies post PCR analysis, allowing direct loading of PCR product on agarose gels following amplification. The GelTrack Loading Dye solution is not included with the sample kit.
AccuStart II PCR ToughMix is stable for 1 year when stored in a constant temperature freezer at -20°C. After thawing, mix thoroughly before using. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing does not affect product performance.
It contains all components, except primers and template.
Ordering information: AccuStart™ PCR ToughMix includes 2X mix containing optimized concentrations of MgCl2, dNTPs, reaction buffer, hot-start DNA polymerase, stabilizers and gel loading dyes. GelTrack Loading Dye includes 50X concentrated mixture of RT-PCR compatible, blue and yellow electrophoresis-tracking dyes.
