Anti-6xHis Mouse Polyclonal Antibody [clone: 33D1.D2.G8]

Supplier: Rockland Immunochemical
200-301-382 200-301-382S
RL200-301-382EA 613.04 CAD
RL200-301-382 RL200-301-382S
Anti-6xHis Mouse Polyclonal Antibody [clone: 33D1.D2.G8]
Antibodies
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion Proteins. As such, anti-6X His/6X His can be used to identify fusion Proteins that contain the 6X His epitope.

Recommended Dilutions: ELISA: 1:20000 - 1:100000; Western Blot: 1:2000 - 1:10000; Immunoprecipitation: 1:200;

Type: Primary
Antigen: 6xHis
Clonality: Polyclonal
Clone: 33D1.D2.G8
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity:
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SPECIFICATIONS

Antigen symbol 6xHis 6xHis
Antigen name 6X HIS Affinity Tag 6X HIS Affinity Tag
Environmentally Preferable
Antigen synonyms Histidine Epitope Tag|His Tag|Histidine Tag Histidine Epitope Tag|His Tag|Histidine Tag
Antibody type Primary Tag
Clonality Polyclonal Monoclonal
Conjugation Unconjugated Unconjugated
Clone 33D10.D2.G8 33D10.D2
Reactivity
Host Mouse Mouse
Isotype IgG1 IgG1 kappa
Western blot Yes Yes
ELISA Yes Yes
ImmunoChemistry Yes
ImmunoPrecipitation Yes
Flow cytometry Yes
ChIP Yes
Immunogen This His antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Sequence HHHHHH
Format Antibody fraction
Form Liquid (sterile filtered)
Purification affinity-purified
Concentration 1.0 mg/ml by UV absorbance at 280 nm
Cross adsorption No
Storage buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide,
Storage temperature –20 °C
Shipping temperature Dry Ice

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