Quantifoil® Holey Carbon Films, Electron Microscopy Sciences

Supplier: Electron Microscopy Sciences
Q2100NR665 Q3100NR2 Q4100CMA Q450CR-14 Q310CR-06 Q210CR-06 Q250CR-14 Q3100AR-12 Q2100NR3 Q4100NR665 Q4100CR665 Q4100NR21.2 Q2100NR105 Q225CR-14 Q425CS7 Q250-NR1 Q2100CR1 Q4100CR105 Q3100CR175 Q325AR1 Q425CR-4 Q325NR2 Q350NR-4 Q220AR-06 Q225CR-520 Q4100CR-4 Q2100NR2 Q425NR1 Q3100AR175 Q350NR1.3 Q350NR-35 Q450NR-35 Q250AR-14 Q4100NR1020 Q4100CR175 Q350NMA Q3100AS7 Q250NR-35 Q2100AR-4 Q450AR-4 Q2100NR-12 Q350NR-520 Q425AR-4 Q3100NR665 Q250-CR1.3 Q2100CR5 Q325NR-4 Q250-AR2 Q210AR-06 Q325AR-14 Q3100CR1010 Q3100AR665 Q3100AR6100 Q350NR1 Q425CR-06 Q3100AR2 Q325CR2 Q2100AS7 Q4100NR1 Q4100NR6100 Q410CR-06 Q425AR-14 Q450CR-06 Q4100CR1010 Q2100NR21.2 Q3100NR175 Q250-CMA Q3100NR105 Q225-CR1.3 Q250-NR4 Q2100NR1 Q450CMA Q3100NMA Q4100AR105 Q350CR-14 Q250-CR4 Q4100AR6100 Q225AR-520 Q450NR-520 Q325NR-06 Q350NR-14 Q250NR-14 Q450AR-520 Q4100NR175 Q325NR1 Q225-NR4 Q4100AR-4 Q225-CR4 Q325CR-14 Q425NR-35 Q325CS7 Q250AR-35 Q410AR-06 Q4100AS7 Q325CR1.3 Q2100NR-4 Q425NR1.3 Q225AR-35 Q225CR-35 Q2100CR1010 Q4100NR510 Q210NR-06 Q2100AR510 Q450NMA Q2100CR21.2 Q325CR-35 Q250CR-35 Q4100CR6100 Q3100CR665 Q3100AR3 Q2100AR175 Q420NR-06 Q450NR1 Q250-CS7 Q325NR-14 Q225-AS7 Q320NR-06 Q2100AR5 Q250NR-520 Q250-CR1 Q3100CR2 Q2100AR2 Q3100CR510 Q225-AR2 Q3100AR1010 Q425NR-4 Q350CR1.3 Q350CMA Q2100CR1020 Q220CR-06 Q4100CR1.3 Q425NR-14 Q420AR-06 Q3100NR1 Q450CR-35 Q3100CMA Q450NS7 Q350CR2 Q2100CMA Q2100AR665 Q3100NR-12 Q250-NR1.3 Q450CS7 Q425CR1 Q225NR-14 Q3100NR-4 Q2100NR1020 Q250-CR2 Q425NMA Q350AR1 Q4100NR5 Q250-NS7 Q3100AR1 Q325NR-520 Q450NR-06 Q350AR-06 Q350CR-520 Q250AR-520 Q250-AS7 Q2100NR5 Q450AR-06 Q450CR1.3 Q4100CR-12 Q4100NR3 Q2100CR3 Q350CS7 Q350NR-06 Q4100AR5 Q225-CR1 Q250CR-06 Q3100AR5 Q4100CR1020 Q3100AR510 Q425NR-520 Q220NR-06 Q4100NR-4 Q320AR-06 Q4100NR1.3 Q325AR-06 Q250-NR2 Q310NR-06 Q4100CR2 Q225-CR2 Q425AS7 Q225AR-14 Q3100CR-12 Q4100CR510 Q325AR-520 Q225NR-06 Q325NR-35 Q325CR-520 Q3100CR3 Q225NR-520 Q3100AR-4 Q325AS7 Q350AR2 Q250AR-06 Q2100AR1020 Q250CR-520 Q4100AR665 Q3100CR1020 Q3100CR-4 Q4100CS7 Q4100CR1 Q4100NR-12 Q350AR-35 Q425AR1 Q450NR2 Q350CR-35 Q3100NS7 Q425CR2 Q2100NR510 Q2100AR1010 Q3100CS7 Q2100NR1010 Q450AS7 Q3100NR510 Q225CR-06 Q420CR-06 Q325AR2 Q450CR2 Q325CR-06 Q3100NR6100 Q225NR-35 Q3100CR1 Q2100CR2 Q350CR-4 Q2100AR105 Q4100AR2 Q450CR1 Q250-AR1 Q2100CR1.3 Q3100CR105 Q4100NR1010 Q2100CS7 Q3100CR1.3 Q3100CR5 Q3100NR1020 Q3100NR5 Q410NR-06 Q4100AR3 Q3100AR1020 Q2100CR6100 Q350CR1 Q450NR1.3 Q450AR-14 Q4100NR2 Q350AR-4 Q325CR1 Q2100NMA Q325NR1.3 Q310AR-06 Q2100CR510 Q450AR-35 Q4100AR1010 Q2100AR3 Q325NS7 Q4100NS7 Q425NS7 Q3100NR1.3 Q450AR2 Q425CR-35 Q425NR2 Q2100AR1 Q320CR-06 Q350AR-14 Q325CMA Q3100CR21.2 Q325NMA Q225-CS7 Q225-AR1 Q4100CR21.2 Q4100NMA Q2100CR175 Q2100CR-4 Q2100AR6100 Q350AR-520 Q225AR-06 Q250-NMA Q225-CMA Q450CR-520 Q250NR-06 Q325CR-4 Q2100CR105 Q425AR-06 Q425NR-06 Q4100AR-12 Q2100NS7 Q4100NR105 Q225-NMA Q425AR2 Q350CR-06 Q3100NR21.2 Q4100CR3 Q350AS7 Q425CR1.3 Q2100NR1.3 Q350NR2 Q425AR-520 Q450AR1 Q3100CR6100 Q2100AR-12 Q4100AR1 Q450CR-4 Q350NS7 Q2100NR175 Q450NR-4 Q325AR-35 Q450NR-14
76441-016PK 359.01 CAD
76441-016 103306-932 103306-812 103306-934 103306-814 76441-130 100491-222 102773-330 102773-450 102773-212 102773-454 102773-332 102773-210 102773-452 102773-338 102773-216 102773-458 102773-336 103306-930 103306-810 102773-214 102773-456 102773-218 103306-826 103306-828 103306-822 103307-052 103306-824 103307-054 100491-212 100491-218 76441-122 100491-216 102773-220 102773-462 102773-340 102773-460 102773-466 102773-224 102773-344 100491-220 102773-222 102773-464 102773-342 102773-228 102773-348 102773-226 102773-468 102773-346 103306-838 76441-114 100491-208 103306-834 103306-836 100491-202 102773-480 100491-206 100491-204 102773-352 102773-472 102773-350 102773-470 102773-356 102773-234 102773-476 102773-354 102773-232 102773-474 76440-556 102773-238 102773-358 102773-236 102773-478 103306-832 103306-844 103305-800 102773-370 102773-490 102773-484 102773-362 102773-482 102773-240 102773-360 76440-308 102773-488 102773-246 103305-802 102773-366 76440-664 102773-486 102773-364 103306-840 102773-248 103306-842 76440-300 102773-368 103307-020 103307-024 103307-022 102773-260 102773-380 103307-026 102773-374 102773-252 102773-494 102773-372 102773-250 102773-492 102773-136 102773-378 102773-498 102773-256 102773-134 102773-376 102773-496 102773-254 103306-576 103306-578 102773-138 102773-258 103306-574 103306-904 103306-906 103306-900 103306-902 103307-010 102773-150 103307-016 102773-392 103306-908 102773-270 103307-014 102773-390 102773-264 102773-142 102773-384 103307-018 102773-140 102773-382 102773-268 102773-388 102773-146 76440-248 102773-266 102773-386 102773-144 103306-346 102773-148 103306-344 103306-910 103307-042 103306-912 103307-040 103307-046 103307-044 103306-918 102773-282 102773-160 102773-280 103307-048 102773-396 102773-154 102773-274 102101-506 102773-152 102773-394 102773-272 102773-158 102773-278 102773-156 102773-398 102773-276 103306-356 103306-354 103306-926 103306-928 103307-030 103306-922 103306-924 103307-034 103307-032 103307-038 102773-172 102773-292 102773-170 103307-036 102773-290 102773-286 102773-164 102773-284 102773-162 102773-168 102773-288 102773-166 102101-512 102101-510 103306-920 76440-292 103306-418 102773-182 102773-180 102773-176 102773-296 102773-174 102773-294 76440-610 102773-178 102773-298 103306-896 103306-898 103306-892 103306-894 103306-410 102773-190 102773-194 76440-716 102773-192 76440-724 102773-186 102773-184 76440-962 102773-188 103306-422 102773-502 102773-500 103306-420 102773-504 103307-002 103307-000 103307-006 76440-708 103307-004 102773-198 102773-196 103307-008 76441-070 102773-400 102773-404 102773-402 102773-408 76440-140 102773-406 100491-268 100491-272 100491-270 102773-410 102773-414 102773-412 102773-418 102773-416 103305-820 100491-258 103305-822 100491-256 103305-828 103305-824 102773-422 100491-264 102773-300 102773-420 103305-826 102773-426 102773-304 102773-424 102773-302 102773-308 102773-428 103305-830 103305-832 100491-244 100491-248 102773-312 100491-254 102773-432 103305-834 102773-310 100491-252 102773-430 103306-994 102773-316 103306-996 102773-314 102773-434 76440-194 103306-992 102773-318 102773-438 103306-408 103306-404 103306-406 100491-234 100491-238 102773-440 102773-202 102773-444 102773-322 100491-242 102773-442 102773-200 102773-320 100491-240 102773-448 102773-206 102773-326 102773-204 102773-446 102773-324 102773-208 102773-328
Quantifoil® Holey Carbon Films, Electron Microscopy Sciences
Graticules, Reticles and Grids
Holey carbon film for electron microscopy or low-energy electron point source microscopy.

  • Offered with circular and square, orthogonal arranged holes
  • Hole sizes from 0.6, 1, 1.2, 2, 3, 3.5, 5, 6, 10 and 17.5 µm
  • 200, 300, and 400 mesh copper and nickel grids
  • Carbon film is about 10 to 12 nm thick

The surface properties of QUANTIFOIL® holey carbon support film, especially the wetting properties, may have to be adapted according to one's particular requirements. Untreated aging QUANTIFOIL® tends to be hydrophobic. Hydrophillicity of the foil can be achieved by glow discharging in residual air or by metal coating.

QUANTIFOIL® in low-energy electron point source (LEEPS) microscopy. Quantifoil® with a regular pattern is required in order to be able to distinguish an object, which is spanned over a hole. An object cannot be discriminated from the support in the case of conventional holey support film. (H.W. Fink & C. Schonenberger, University of Basel, used QUANTIFOIL® for the measurement of electrical current through DNA molecules)

The foil is ~12 nm thick and mounted on either copper, nickel or gold grids with either square or round holes of different sizes. Holey films with 2 µm round holes are used at magnifications between 30000× and 40000×.

QUANTIFOIL® with circular holes is mainly used in cryo-EM. The roundness of the holes is advantageous with respect to the formation of an ice layer of constant thickness. The hole size that is chosen depends on the magnification used, and on whether or not one wishes to include support film in the image. The latter makes assessment of the image quality easier, because the power spectrum of a foil is stronger than that of unsupported ice. The QUANTIFOIL® types with small holes (1.2 µm) were designed for magnifications around and higher 50000×. At magnifications between 30000× and 40000× we recommend the QUANTIFOIL® types with 2 µm holes. Larger holes (3.5 µm, 5 µm) are suitable at magnification around 20000×.

QUANTIFOIL® R 3.5/1 may be preferred over foils with smaller holes if the carbon film should be outside the frame of the image. This option can be desirable in the case of extended objects, such as filamentous objects, for example.
QUANTIFOIL® R 1/4 may be preferred over R 1.2/1.3, when an increased tolerance with respect to the position of beam, and a larger beam diameter are desired, such as in the case of automated image acquisition.
QUANTIFOIL® R 0.6/1 Hole size is 0.5 µm. Space between holes is 1 µm. Center to center is 1.6 µm (hole size may be as large as 1 µm).
QUANTIFOIL® R 5/20 Hole size is 5 µm. Space between holes is 20 µm. Center to center is 25 µm.
QUANTIFOIL® R 2/4 may be preferred over R 2/2, when an increased tolerance with respect to the position of beam, and a larger beam diameter are desired, such as in the case of automated image acquisition.
QUANTIFOIL® R 2/2 Holey films with 2 µm circular holes are used at magnifications between 30000× and 40000×.
QUANTIFOIL® R 2/1 has more open area than R 2/2. It is used when focusing is carried out on the edge of a hole burnt in the ice in a neighboring hole instead of on the foil adjacent to the hole.
QUANTIFOIL® R 1.2/1.3 A foil with ~1.2 µm circular holes and a spacing of ~2.5 µm between the holes. This type is used at magnifications around 50000×.

QUANTIFOIL® with square holes and relatively narrow bars can be used in electron microscopy to support a thin carbon film, which by itself is too fragile to span a grid square. The QUANTIFOIL® types with square holes constitute an optimum between a maximum of open area on the one hand, and mechanical stability on the other hand.
QUANTIFOIL® S 7/2 constitutes an optimum between a maximum of open area on the one hand, and mechanical stability on the other hand.

QUANTIFOIL® Multi A is a holey film, in which a pattern of various hole sizes, shapes and arrangements is repeated. In addition to circular holes, the pattern includes oval-shaped ones which appear round at high tilt angles (~70°). The circular holes have diameters of 1 µm, 1.4 µm, and 2 µm. The elliptical holes measure 1×4 µm and 2×8 µm (along the shortest and longest axes, respectively).

QUANTIFOIL® with Hexagonal Geometry is meant for slot grids. It was especially designed for supporting serial thin sections. It offers an optimum between mechanical stability on the one hand and background-free area on the other hand. The foil is thin enough to allow those parts of the sections that lie on the bars to be interpreted. In this way, the information in the sections can be interpreted to the maximum. QUANTIFOIL® Hex 15 A foil with hole size of 26 µm (diameter of inscribed circle) and a repeating distance of 41 µm, the side length of the holes and the bar width are 15 µm.

Ordering information: QUANTIFOIL® is generally delivered as a carbon foil; it can be reinforced with plastic film. The standard thickness for the carbon foil is 12 nm; other thicknesses between 10 and 25 nm can be made upon request.

Packaging: The grids are packed with their coated side toward the center of the grid box.
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