Hematoxylin modified solution Gill III used in nuclear staining, Sigma-Aldrich®

Supplier: MilliporeSigma
Danger

Synonyms: HydroxybrasilinDcH-48Natural Black I

1.05174.0500 1.05174.2500 1.05174.0500 1.05174.1000 1.05174.2500 1.05174.1000
CA1.05174.0500EA 82.74 CAD
CA1.05174.0500 CA1.05174.2500 EM1.05174.2500 EM1.05174.0500 CA1.05174.1000 EM1.05174.1000
Hematoxylin modified solution Gill III used in nuclear staining, Sigma-Aldrich®
Hematoxylin staining solutions

Hematoxylin solution modified acc. to Gill III


In hematoxylin-eosin staining (H&E), the most widely used histological staining technique, nuclear staining is achieved using ready-to-use staining solutions, such as Mayer’s hemalum solution or Hematoxylin solution modified according to Gill III. These solutions do not need to be filtrated prior use and the dye is oxidized in a controlled manner, which renders the results robust and allows a brilliant differentiation of the stained structures. Counterstaining of i.e. for proteins, collagen, keratin, intercellular substances. can be performed with alcoholic or aqueous Eosin Y solution 0.5 %. A 500 ml bottle is sufficient for 1500 up to 2500 slides. It is registered as IVD and CE product

Formula: C₁₆H₁₄O₆
MW: 302.28 g/mol
Density: 1.07 g/cm3 (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00078111
CAS Number: 517-28-2

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Specification Test Results

Suitability for microscopy passes test
Nuclei dark blue to dark violet
Cytoplasm pink to red
intercellular substances pink to red
Erythrocytes yellow to orange
Eosinophilic cytoplasm pink

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