BacMagic™-2 DNA Kit, MilliporeSigma

Supplier: MilliporeSigma

72157-3 70967-6 70967-6 70967-3 70967-5 70967-5 72157-3 70967-3

CA80018-132EA 2216.7 CAD

CA80018-132 80018-132 80511-356 CA80511-356 80017-194 CA80017-194 80511-360 CA80511-360

BacMagic™-2 DNA Kit, MilliporeSigma

Protein Expression Protein Expression Systems

The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells

BacMagic-2 DNA is derived from an AcNPV genome,
contains an ORF 1629 deletion which prevents nonrecombinant virus from replicating in insect cells, and contains a deletion of the nonessential chitinase (chiA) gene, to improve production of secreted and membrane-targeted proteins. BacMagic-2 DNA have an additional deletion of the v-cath gene, a cathespsin-like cysteine protease, resulting in significantly improved quality and yield for most target proteins.

The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

Ordering information: The BacMagic-2 DNA Kit contains 0.5 µg of the BacMagic-2 DNA, 50 µL of the Insect GeneJuice® Transfection Reagent, and 2 µg of a positive control plasmid to monitor co-transfection efficiency.

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