BL21 Competent Cells, Expression Strains, MilliporeSigma

Supplier: MilliporeSigma
69449-3 69449-4 69450-3 69450-4 69451-3 69451-4
CA80030-318EA 378.86 CAD
CA80030-318 CA80030-320 CA80030-322 CA80030-324 CA80030-326 CA80030-328
BL21 Competent Cells, Expression Strains, MilliporeSigma
Competent Cells
A common method for transformation of plasmids into E

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lac UV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilising pET recombinants encoding target proteins that affect cell growth and viability.

The standard volume per tube is 0,2 ml,More Product Information
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