KOD Hot Start DNA Polymerase®, MilliporeSigma

Supplier: MilliporeSigma

71086-5 71086-3 71086-4

CA60801-146EA 257.03 CAD

CA60801-146 CA80511-384 CA80511-386

KOD Hot Start DNA Polymerase®, MilliporeSigma

Nucleic Acid Reagents End-point PCR Enzymes and Kits

KOD Hot Start DNA Polymerase® is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3′→5′ exonuclease activities at ambient temperatures (Mizuguchi 1999)

KOD Hot Start combines the high fidelity, fast extension speed, and outstanding processivity of KOD with the high specificity of an antibody-mediated hot start. Non-specific amplification is reduced because mispriming events that can occur during setup and initial temperature increase are avoided. In addition, primer degradation during setup at room temperature due to exonuclease activity is effectively inhibited. KOD Hot Start DNA Polymerase generates blunt-ended PCR products suitable for cloning with the Novagen Perfectly Blunt® and LIC Vector Kits. This is a heat-activatable form of KOD DNA Polymerase for increased specificity and convenient PCR setup.

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