M13mp18 RF I DNA, New England Biolabs

Supplier: New England Biolabs

M13mp18 RF I DNA - 10 µg

M13mp18 RF I DNA - 10 µg

N4018S N4018S

CA101228-156EA 144 CAD

CA101228-156 101228-156

M13mp18 RF I DNA, New England Biolabs

Vectors, Plasmids and Libraries

M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13.

This phage vector contains single HindIII, SphI, SbfI, PstI, SalI (AccI/ HincII), XbaI, BamHI, SmaI (XmaI), KpnI (Acc65I), SacI and EcoRI sites within the β-Galactosidase gene. When a fragment of DNA is inserted into one of these sites, the β-Galactosidase gene is inactivated, providing selection for clones on the appropriate indicator plate. DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful. The phage M13mp18 is propagated in E. coli ER2738. The replicative form of DNA is isolated from infected cells and purified by a standard plasmid purification procedure. The final preparation is tested for its suitability as a vector.

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