Bst 2.0 WARMSTART® DNA Polymerase, New England Biolabs

Supplier: New England Biolabs (NEB)

M0538L M0538M M0538S
CA102500-034EA 542 CAD
CA102500-034 CA102500-036 CA102500-038
Bst 2.0 WARMSTART® DNA Polymerase, New England Biolabs
Nucleic Acid Reagents End-point PCR Enzymes and Kits

Bst 2.0 WarmStart DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45°C.


The aptamer rapidly releases the Bst 2.0 WarmStart DNA Polymerase above 45°C and therefore no special activation step is needed to activate the polymerase. Bst 2.0 WarmStart DNA Polymerase contains 5´→3´ DNA polymerase activity and strong strand-displacement activity but lacks 5´→3´ exonuclease activity. Bst 2.0 WarmStart DNA Polymerase displays improved amplification speed, yield, salt tolerance, and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.

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