Antarctic Phosphatase

Supplier: New England Biolabs (NEB)

Antarctic Phosphatase - 1,000 units

Antarctic Phosphatase - 1,000 units

M0289L M0289S

CA101228-348EA 499 CAD

CA101228-348 CA101228-350

Antarctic Phosphatase

Enzymes

Antarctic Phosphatase catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters.

Antarctic Phosphatase also hydrolyzes ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). Antarctic Phosphatase can be used in many molecular biology applications, such as the removal of phosphorylated ends of DNA and RNA, for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA. The enzyme acts on 5´ protruding, 5´ recessed and blunt ends. Antarctic Phosphatase may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing. The enzyme is completely and irreversibly inactivated by heating at 70°C for 5 minutes, thereby making removal of Antarctic Phosphatase prior to ligation or end-labeling unnecessary.

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