HiTrap Columns, DEAE Sepharose FF, Cytiva

Supplier: Cytiva

HiTrap Columns, DEAE Sepharose FF, Cytiva

HiTrap Columns, DEAE Sepharose FF, Cytiva

HiTrap Columns, DEAE Sepharose FF, Cytiva

HiTrap Columns, DEAE Sepharose FF, Cytiva

HiTrap Columns, DEAE Sepharose FF, Cytiva

HiTrap Columns, DEAE Sepharose FF, Cytiva

HiTrap Columns, DEAE Sepharose FF, Cytiva

HiTrap Columns, DEAE Sepharose FF, Cytiva

HiTrap™

17-5055-01 17-5154-01 17-5154-01 17-5055-01

CA95056-084LPK 458.49 CAD

95056-084 CA95056-084L 95056-100 CA95056-100L

HiTrap Columns, DEAE Sepharose FF, Cytiva

Chromatography Columns

HiTrap DEAE FF is prepacked with DEAE Sepharose Fast Flow, and is a weak anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Convenient and affordable for fast, easy ion exchange separations either alone or connected in series
The industry standard for ion exchange chromatography
High flow rates and good scale-up potential
Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient
Predictable scale-up

The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose 4 Fast Flow (high sub) has a diethylaminopropyl group attached.

Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

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