GSTrap FF Columns, Cytiva

Supplier: Cytiva

Glutathione Sepharose™ media

Ni Sepharose 6 fast flow

Collection of Chromatography media and HiTrap columns

Glutathione Sepharose™ media

Ni Sepharose 6 fast flow

Collection of Chromatography media and HiTrap columns

17-5130-01 17-5130-02 17-5131-01 17-5131-02

CA95055-894LPK 948.03 CAD

CA95055-894L CA95056-092L CA95056-094L CA95056-096L

GSTrap FF Columns, Cytiva

Chromatography Columns

GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

Fast and simple one-step purification of GST-tagged proteins
Mild elution conditions preserving protein antigenicity and function
Easy scale-up by connecting the columns in series
Sepharose 4 Fast Flow provides good flow properties

GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione.

The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.

The resin, Glutathione Sepharose 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.

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