HiTrap Columns, rProtein A FF, Cytiva

Supplier: Cytiva
17-5079-02 17-5080-01 17-5079-01 17-5080-02
CA95055-868LPK 1029.88 CAD
CA95055-868L CA95056-008L CA95056-088L CA95056-090L
HiTrap Columns, rProtein A FF, Cytiva
Chromatography Columns
HiTrap rProtein A FF columns are prepacked with rProtein A Sepharose Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.

  • Quick, convenient preparative purification of monoclonal antibodies
  • Well-established protein A-based resin used in many approved MAb processes
  • Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA design

Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.

The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
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