PerfeCTa® qPCR ToughMix®, QuantaBio

Supplier: Quantabio
PerfeCTa® qPCR ToughMix®
95138-012 95112-250 95138-012 95113-012 95138-05K 95139-012 95112-05K 95138-05K 95139-05K 95112-012 95114-05K 95114-250 95113-05K 95112-05K 95114-05K 95112-012 95113-012 95139-012 95114-250 95139-05K 95112-250 95140-012 95113-05K 95140-05K 95114-012 95140-05K 95140-012 95114-012
CA89409-154EA 6235.12 CAD
CA89409-154 89409-146 89409-148 97065-958 CA97065-968 CA97065-954 97065-968 97065-966 CA97065-970 97065-962 97065-960 89409-152 CA89409-140 89409-154 CA89409-142 CA89409-146 CA89409-148 CA97065-958 CA97065-956 97065-956 CA97065-966 97065-954 CA97065-962 CA97065-960 97065-970 89409-140 89409-142 CA89409-152
PerfeCTa® qPCR ToughMix®, QuantaBio
Nucleic Acid Reagents qPCR/RT-qPCR Enzymes and Kits
PerfeCTa qPCR ToughMix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues

  • ToughMix formulation neutralize PCR inhibitors to ensure reliable assay performance with a variety of starting materials
  • Exceptional reagent stability (uninhibited after 20X freeze-thaw or 30 days at 22 °C) and anti-foam technology for efficient vortex mixing
  • Easy-to-use 2X concentrated mastermixes with AccuVue™ plate loading dye and optimized passive reference dye

A key component of PerfeCTa qPCR ToughMix is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. PerfeCTa qPCR ToughMix delivers exceptional performance with either fast or conventional PCR cycling protocols.

PerfeCTa qPCR ToughMix is compatible with many Real-Time PCR Instruments, such as the Roche LightCycler™ 480, Qiagen/Corbett Rotor-Gene® Q, Qiagen/Corbett Rotor-Gene® 6000, Qiagen/Corbett Rotor-Gene® 3000, Eppendorf Mastercycler® ep realplex, Eppendorf Mastercycler® ep realplex2 s, Cepheid SmartCycler®, Bio-Rad CFX96™, Bio-Rad CFX384™, Bio-Rad/MJ Opticon®, Bio-Rad/MJ Opticon 2, Bio-Rad/MJ Chromo4™, Bio-Rad MiniOpticon™, Illumina Eco qPCR, Thermo Scientific PikoReal Cycler, BioRad iCycler iQ™, BioRad MyiQ™ and BioRad iQ™5.

PerfeCTa qPCR ToughMix is stable for 2 years when stored in a constant temperature freezer at –20°C. For convenience, it may be stored unfrozen at 2 to 8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing does not affect PCR performance.

It is a versatile and robust real-time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa qPCR ToughMix contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

Ordering information: PerfeCTa qPCR ToughMix contains 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, AccuVue blue qPCR dye and stabilizers. For research use only. Not intended for any animal or human therapeutic or diagnostic use. Please consuly the Material Safety Data Sheet for information regarding hazards and safe handling practices.
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