DNA Marker, High Range, 1000 bp, Thermo Scientific
Supplier: Thermo Scientific Chemicals
DNA ladders are used in gel electrophoresis to determine the size and quantity of testing DNA fragments of genomic, plasmid, and PCR DNA.
- Form: Liquid
- Storage: Store at –20 °C
- Solubility: Soluble in 10 mM Tris-HCl (pH 8.0), 1.0 mM EDTA, 50 mM NaCl
- Contains 8 bands from 1000 to 10000 base pairs: 1000 bp increments from 1000 to 6000 bp, 8000 and 10000 bp
It is commonly used in the studies of plasmid DNA and PCR products. 1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during electrophoresis. 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In NA electrophoresis, five of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in northern and southern blotting. All DNA markers can be stained with ethidium bromide, SYBR® Green, and Nancy-520.
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