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Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

Maintaining Sample Integrity with Aerosol-blocking Tips Liquid Handling Consumables Thermo Fisher Scientific Because of the sensitivity of PCR a variety of factors can compromise the integrity of the reaction. This illustrates the importance of us...

PowerPoint Presentation Comparative Proteomics Kit II: Western Blot Module Comparative Proteomics Kit II: Western Blot Module Instructors Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead...

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The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

Proteomics PROTEIN EXPRESSION ☐ Protein Expression Mammalian Routine Tightly Controlled Ultra-Pure ☐ Prokaryotic Cells Competent Cells Electroporation Induction Reagents PET Vectors for Gateway Cloning ☐ Transfection Insect Cells Mammalian Cells ☐...

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PRODUCT CHECKLIST Equipment ☐ Anaerobic Systems Chambers Indicators Jars ☐ Anesthesia Equipment ☐ Autoclaves/Sterilizers Portable Top-Loading ☐ Blenders ☐ Cell Counters ☐ Centrifuges Benchtop Centrifugal Concentrators Clinical Floor/Ultra Micro/Mi...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

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Introduction Change is hard. It takes work to adapt to a new technology and to get used to something different. It’s especially hard if you have something that already seems to work. This is a big reason people still use film and darkrooms to proc...

LABORATORY CHECKLIST Genomics ☐ Plasmid Purification Accessories Colony Screening Column Regeneration Endotoxin-Free Plasmid Isolation Rapid Plasmid Isolation Standard High Purity Plasmid Isolation Yeast Plasmid Isolation ☐ Genomic DNA Purificatio...

LABORATORY CHECKLIST Genomics ☐ Plasmid Purification Standard High Purity Plasmid Isolation Rapid Plasmid Isolation Endotoxin-Free Plasmid Isolation Yeast Plasmid Isolation Colony Screening Column Regeneration Accessories ☐ Genomic DNA Purificatio...

Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...