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Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

Introduction Change is hard. It takes work to adapt to a new technology and to get used to something different. It’s especially hard if you have something that already seems to work. This is a big reason people still use film and darkrooms to proc...

LABORATORY CHECKLIST Genomics ☐ Plasmid Purification Standard High Purity Plasmid Isolation Rapid Plasmid Isolation Endotoxin-Free Plasmid Isolation Yeast Plasmid Isolation Colony Screening Column Regeneration Accessories ☐ Genomic DNA Purificatio...

Proteomics PROTEIN EXPRESSION ☐ Protein Expression Mammalian Routine Tightly Controlled Ultra-Pure ☐ Prokaryotic Cells Competent Cells Electroporation Induction Reagents PET Vectors for Gateway Cloning ☐ Transfection Insect Cells Mammalian Cells ☐...

Bad reactions to latex gloves? PROTECT YOURSELF Criteria for Glove Selection When Double Donning in Life Sciences and Laboratory Environments By Don Cronk Regulatory Affairs and Technical Services Manager Ansell Introduction Single-use gloves are ...

LABORATORY CHECKLIST Genomics ☐ Plasmid Purification Accessories Colony Screening Column Regeneration Endotoxin-Free Plasmid Isolation Rapid Plasmid Isolation Standard High Purity Plasmid Isolation Yeast Plasmid Isolation ☐ Genomic DNA Purificatio...

Working with Daphnia Diagrammatic Anatomy of a female Daphnia 1. Frontal organ 16. Carapace 2. Esophagus 17. Filter setae 3. Antennal adductor muscles 18. 2nd and 3rd thoracic appendages 4. Antenna 19. Shell gland 5. Antennal levator muscle 20. Ma...

The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

Azure Biosystems is dedicated to designing instruments that can deliver industry-leading performance across a broad range of laboratory applications without overcomplicating the user experience. We have designed a suite of upgradeable cSeries inst...

Manufacturing CUSTOM CHEMICALS AND SERVICES SUPPORTING SCIENTIFIC ADVANCES FOR HUMAN HEALTH VWR Custom Manufacturing Services2 VWR enables the advancement of science by providing high-quality chemicals and services customized to your product or ma...

Bad reactions to latex gloves? PROTECT YOURSELF Protect yourself from Type I allergies. Introduction Occasionally wearing glove products can cause issues with the health of our skin. This predominantly manifests itself in the form of skin allergie...

PRODUCT CHECKLIST Glassware and plasticware ☐ Beakers* ☐ Bottles Caps/Closures Centrifuge* Environmental Media* Sampling* Storage* Wash ☐ Burets* ☐ Carboys ☐ Cryogenic Vials Cryo Boxes/Racks ☐ Culture Tubes* ☐ Cuvettes* Acrylic Glass Polystyrene ☐...

continued on next page Problem A large non-profi t medical research organization needed to move its primary shipping and receiving operation to an off-site location when the on-site lease was due to expire. Moving an operation of such size would r...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

continued on next page Problem A large non-profi t medical research organization needed to move its primary shipping and receiving operation to an off-site location when the on-site lease was due to expire. Moving an operation of such size would r...

continued on next page SCIENTIFIC SERVICES ENABLE BIOREPOSITORY’S WORKFORCE TRANSFORMATION SUCCESS STORY Scientific Sample Management: Clinical Biorepository CHALLENGE A shift in financial focus resulted in workforce and workload strain at a R&D c...