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Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

Click to edit Master title style Got Protein? Testing the protein content of common foods Bradford Protein Assay Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller...

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TECHNICAL BULLETIN “Pipetting error” is a phrase that is commonly heard in research laboratories. When preparing reagents and master mixes pipetting error is accommodated for. What is not typically discussed or published are the causes and ramific...

continued on next page Problem The pace of research is accelerating. New aggressive discovery timelines mean that scientists face increasing pressure to deliver results quickly. As part of continuous innovation efforts a team at one of VWRCATALYST...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

continued on next page Problem The pace of research is accelerating. New aggressive discovery timelines mean that scientists face increasing pressure to deliver results quickly. As part of continuous innovation efforts a team at one of VWRCATALYST...

Cell culture ☐ Animal and Human Sera Bovine Serum Albumin (BSA) Fetal Bovine Serum (FBS) FBS Alternatives Other Sera ☐ Buffered Salt Solutions DPBS EBSS HBSS Other PBS ☐ Cell Counting Automated Manual Viability Reagents ☐ 3D Cell Culture 3D Cell C...