You Searched For: Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.


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Catalog Number: (CAPI31497)
Supplier: Thermo Scientific
Description: The Thermo Scientific Pierce Activated Peroxidase and Antibody Labeling Kit is designed to prepare and purify HRP-conjugated antibodies with any previously unmodified primary or secondary antibody (IgG).

Supplier: Thermo Scientific
Description: The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.
Catalog Number: (CA71003-152)
Supplier: G-Biosciences
Description: G-Biosciences' CDI Amine Reactive Agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.

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Catalog Number: (CA101447-672)
Supplier: New England Biolabs (NEB)
Description: SNAP-Surface® 488 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells


Catalog Number: (CA101447-756)
Supplier: New England Biolabs (NEB)
Description: BG-GLA-NHS is an amine-reactive building block for the one-step synthesis of SNAP-tag® substrates from amine-containing precursors including proteins, peptides or oligonucleotides


Catalog Number: (CA101447-658)
Supplier: New England Biolabs (NEB)
Description: SNAP-Cell Oregon Green is a photostable green fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells.


Supplier: Biotium
Description: Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for <i>in vivo</i> imaging.

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Supplier: Biotium
Description: CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labeling reactions are also available.

Catalog Number: (77461-682)
Supplier: AAT BIOQUEST INC
Description: Helixyte™ iFluor® 488 Nucleic acid labeling dye is a key member of our enabling Helixyte™ nucleic acid labeling and conjugation technology.


Catalog Number: (77462-024)
Supplier: AAT BIOQUEST INC
Description: XFD750, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 750, a trademark of ThermoFisher.


Catalog Number: (CAPI20160)
Supplier: Thermo Scientific
Description: The Thermo Scientific Pierce RNA 3' End Biotinylation Kit is optimized for labeling the 3-prime terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions.

Supplier: G-Biosciences
Description: G-Biosciences' Carboxyl Coupling Resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm

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Supplier: Thermo Scientific
Description: Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.

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Supplier: G-Biosciences
Description: Fluorescein Isothiocynate (FITC) is the simplest and most commonly used fluorescent dye for labeling of proteins, peptides, and antibodies

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Supplier: G-Biosciences
Description: Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin.

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Supplier: G-Biosciences
Description: G-Biosciences' avidin is a glycoprotein with approximately 10% of its total mass comes from carbohydrates

SDS

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