You Searched For: Agarose and Acrylamide

Agarose and acrylamide are the most commonly used electrophoresis gels for their versatility with buffers and ability to generate reproducible results. Agarose typically runs horizontal tests to resolve large DNA fragments while acrylamide runs vertical separations for shorter nucleic acids. Easy to handle, the gels are chosen based on the high resolution desired and the samples. The concentration or ratio of the agarose and acrylamide will determine the overall working porosity.

Agarose and acrylamide are the most commonly used electrophoresis gels for their versatility with buffers and ability to generate reproducible results. Agarose typically runs horizontal tests to resolve large DNA fragments while acrylamide runs vertical separations for shorter nucleic acids. Easy to handle, the gels are chosen based on the high resolution desired and the samples. The concentration or ratio of the agarose and acrylamide will determine the overall working porosity.


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Supplier: VWR
Description: Agarose SFR™ (Super Fine Resolution) is a high resolution sieving agarose with excellent clarity. DNA bands differing in size by 2% can be resolved in the range of 200 bp to 1000 bp.
Supplier: VWR
Description: A quality general application agarose that provides good resolution and is cost-effective for high volume users.
Supplier: Ward's Science
Description: Prepared with TBE buffer, these gels are easy to use — simply melt them in a microwave oven or water bath then pour them into a casting tray. The non-reusable gels have a one-year shelf life and should be stored at room temperature.

SDS

Supplier: VWR
Description: An extensive line of acrylamide and bis-acrylamide pre-weighed powder blends, premixed stock solutions and ready-to-use solutions for customized polyacrylamide gel electrophoresis of proteins and PAGE of nucleic acids. Ultra-pure (>99.9%) acrylamide and bis-acrylamide powders provide the flexibility to prepare solutions having concentrations and ratios for all electrophoresis applications. Liquid blends minimize the handling of neurotoxic acrylamide.
Supplier: VWR
Description: Agarose I™ is for routine nucleic acid analytical/preparative applications.
Catalog Number: (97064-840)
Supplier: VWR
Description: VWR® Cyclo-Pure™ Agarose Gel Extraction Kit is a fast, spin column-based kit for the quick isolation of DNA fragments (40 bp - 100 kb) from agarose slices. In just 10 minutes, ultra-pure DNA can be extracted that is ready-to-use in restriction enzyme digestion, labeling, ligation, transformation, in vitro transcription and sequencing protocols. All reagents are room temperature stable.

Supplier: HARDY DIAGNOSTICS CA
Description: CulGenex™ Agarose LE, Molecular Biology Grade is recommended for use in the preparation of an electrophoresis gel for the analysis of nucleic acids and proteins
Catalog Number: (CAPI17856)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce Agaroses are highly purified and carefully blended formulations of low-melting agarose that provide uniform lot-to-lot consistency for preparation of electrophoresis gels.

Supplier: Edvotek
Description: Ideal for student skill practice.

Supplier: IBI Scientific
Description: 3:1 Super-Sieve Agarose is designed to have a larger gel network than IBI Ultra Sieve Agarose and is recommended for use in the separation of DNA fragments smaller than 1500bp

Supplier: Biotium
Description: Ultrapure molecular biology grade agarose pre-coated with GelGreen® Nucleic acid gel stain for convenient and safer preparation of precast gels.

Environmentally Preferable

Catalog Number: (CA1710)
Supplier: MilliporeSigma
Description: OmniPur® acrylamide bis-acrylamide, 29:1, 40% solution, is suitable for nucleic acid and protein separation sequencing. Premixed solutions offer standard ratios of acrylamide to bis-acrylamide for convenience and safety. The hazard of handling powdered acrylamide is eliminated with the 40% solution in water. Reconstitute with deionized H₂O to desired final percentage.

SDS


Catalog Number: (470111-860)
Supplier: Edvotek
Description: Provides clearer DNA bands and more resilient gel (20 g—40 standard gels).


Catalog Number: (CAPI20395)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce Jacalin Agarose is effective for specific affinity purification of human IgA from serum or colostrum or efficient removal of contaminating IgA from IgG samples.

Supplier: Thermo Scientific
Description: Thermo Scientific Pierce NHS-Activated Agarose is a high-quality, amine-reactive, beaded-agarose resin for rapid and stable immobilization of proteins, peptides and other ligands via primary amines.
Catalog Number: (76414-950)
Supplier: Biotium
Description: Ultrapure molecular biology grade agarose pre-coated with GelRed® Nucleic Acid Gel Stain for convenient and safer preparation of precast gels.

Environmentally Preferable


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