BL21 Competent Cells, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

230280 200131 200132 200133 230130 230132 230134 230240 230245 230255

CA76193-370PK 383.25 CAD

CA76193-370 CA97066-330 CA99900-860 CAST200131 CAST200132 CAST200133 CAST230130 CAST230132 CAST230134 CAST230240 CAST230245 CAST230255 CAST230275

BL21 Competent Cells, Agilent Technologies

Competent Cells

BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction.

High-level protein expression and easy induction
Can be used with non-T7 RNA polymerase protein expression systems
Allows the tightest control of protein expression for extremely toxic proteins
Suitable for expressing proteins from all species, including mammals
Ideal for difficult protein expression, especially when codon bias is a problem
Increases transformation efficiency with Hte phenotype
Derived from Agilent’s high performance BL21-Gold competent

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BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction.

High-level protein expression and easy induction
Can be used with non-T7 RNA polymerase protein expression systems
Allows the tightest control of protein expression for extremely toxic proteins
Suitable for expressing proteins from all species, including mammals
Ideal for difficult protein expression, especially when codon bias is a problem
Increases transformation efficiency with Hte phenotype
Derived from Agilent’s high performance BL21-Gold competent cell line

BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction. The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems. BL21 has the tightest control of protein expression for extremely toxic proteins.

Agilent BL21-CodonPlus competent cells dramatically improve protein expression in E. coli by overcoming codon bias. Codon bias occurs when forced high-level expression of a gene containing codons rarely expressed in E. coli depletes internal tRNA pools. This often results in poor protein synthesis, early termination of the polypeptide chain, or misincorporation of amino acids into the expressed protein. BL21-CodonPlus competent cells are derived from Agilent’s high performance BL21-Gold competent cell line. These cells enable efficient high-level expression of heterologous proteins in Escherichia coli.;Overcoming codon bias saves time and labor by eliminating the need for site-directed mutagenesis or for expressing the protein in a eukaryotic expression system. L21-CodonPlus competent cells are derivatives of BL21-Gold cells that have been engineered to include extra copies of genes that encode tRNAs for these rare E. coli codons. The additional availability of these tRNAs facilitates high level expression of many heterologous recombinant genes in BL21-CodonPlus competent cells. Efficient production of heterologous proteins in E. coli is frequently limited by the rarity of certain tRNAs that are abundant in the organisms from which the heterologous proteins are derived. Forced high-level expression of heterologous proteins can deplete the pool of rare tRNAs and stall translation.

BL21-CodonPlus competent cells also feature the Hte phenotype present in Agilent’s highest efficiency competent cell strain, XL10-Gold. The presence of the Hte phenotype increases the transformation efficiency of the cells. In addition, the gene that encodes endonuclease I (endA), an enzyme that rapidly degrades plasmid DNA isolated by most miniprep procedures, has been inactivated in these cells. These two features enable direct cloning of many protein expression constructs.

BL21-gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene encoding endonuclease I (endA) inactivated. Together, these two features allow direct cloning for most protein expression constructs. By cloning directly in the strain, you can save two days of work normally spent on subcloning procedures. The DE3-derivatives of BL21 contain the T7 RNA polymerase gene controlled by the lacUV5 promoter. This all-purpose derivative yields high-level expression and provides easy induction. Use this derivative with nontoxic proteins.

Caution: For research use only. Not for use in diagnostic procedures.

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Environmentally Preferable
Genotypes	E. coli B F^– ompT hsdS(r_B^– m_B^–) dcm+ Tet^r gal λ (DE3) endA Hte metA::Tn5(Kan^r) [argU ileY leuW Cam^r]	E. coli B F^– ompT hsdS(r_B^– m_B^–) dcm+ Tet^r gal endA Hte [argU proL Cam^r]		E. coli B F^–ompT hsdS(rB ^–mB^–) dcm+ Tet^r gal endA Hte [argU ileY leuW Cam^r]

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Description	Size	Features	Supplier No.	VWR Catalog Number	Unit	Price >
BL21 competent cells	5×0.2 ml	For extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase or use with non-T7 polymerase-based systems	200133	CAST200133	Each	Retrieving
Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
BL21(DE3) competent cells	5×0.2 ml	For non-toxic proteins, encodes T7 RNA polymerase under the control of the lacUV5 promoter. Use with Variflex or Affinity pCAL vectors.	200131	CAST200131	Each	Retrieving
Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing. Certificates
BL21(DE3) pLysS competent cells	5×0.2 ml	For both toxic and non-toxic proteins, pLysS codes for T7 lysozyme, a T7 RNA polymerase inhibitor. Use with pET or pCAL vectors.	200132	CAST200132	Each	Retrieving
Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing. Certificates
BL21-Gold competent cells	10×0.1 ml	For extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase or use with non-T7 polymerase-based system. Efficiency is ≥1×10⁸ cfu/µg of supercoiled DNA.	230130	CAST230130	Each	Retrieving

BL21-Gold (DE3) competent cells	10×0.1 ml	For non-toxic proteins, encodes T7 RNA polymerase under the control of the lacUV5 promoter. Use with Variflex or Affinity pCAL vectors. Efficiency is ≥1×10⁸ cfu/µg of supercoiled DNA.	230132	CAST230132	Each	Retrieving
Certificates
BL21-Gold (DE3) pLysS competent cells	10×0.1 ml	For both toxic and non-toxic proteins, pLysS codes for T7 lysozome, a T7 RNA polymerase inhibitor. Use with Variflex or Affinity pCAL vectors. Efficiency is ≥1 x 10⁸ cfu/ µg of supercoiled DNA.	230134	CAST230134	Each	Retrieving

BL21-Codonplus -RIL competent cells	10×0.1 ml	For extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase, 1×10⁷ cfu/µg of supercoiled DNA.	230240	CAST230240	Each	Retrieving
Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
BL21-Codonplus (DE3)-RIL competent cells	10×0.1 ml	Encodes T7 RNA polymerase under control of the lacUV5 promoter for easy protein expression, Use with pET or pCAL vectors, 1×10⁷ cfu/µg of supercoiled DNA	230245	CAST230245	Each	Retrieving
Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing. Certificates
BL21-Codonplus (DE3)-RP competent cells	10×0.1 ml	Encodes T7 RNA polymerase under control of the lacUV5 promoter for easy protein expression, Use with pET or pCAL vectors, 1×10⁷ cfu/µg of supercoiled DNA.	230255	CAST230255	Each	Retrieving
Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing. Certificates
BL21-Codonplus (DE3)-RIPL competent cells	10×0.1 ml	These cells contain extra copies of rare E. coli argU, ileY, leuW, proL tRNA genes which corrects for codon bias and dramatically improves expression of sequences from other organisms.	230280	CA76193-370	Pack of 10	Retrieving
Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.

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