Pierce™ Pro-Matrix™ Protein Refolding Kit, Thermo Scientific

Supplier: Thermo Scientific

89867
CAPI89867EA 1693.22 CAD
CAPI89867
Pierce™ Pro-Matrix™ Protein Refolding Kit, Thermo Scientific
Protein Purification Protein Purification Resins

The kit is designed to simplify the development of a high-yield/high-concentration refolding protocol by using components that have the most significant and general utility in order to limit conditions.


  • Conditions and components examined are limited to those having the most significant and general utility as folding buffers
  • Three-level matrix design significantly reduces the amount of secondary optimisation required and increases the ease of data interpretation
  • Allows refolding experiments to be customized to the target protein; known positive and negative interactions between buffer components are addressed, minimising unnecessary analyses
  • Reagents are formulated using stringent standards so that consistent results are attained


It includes nine high-purity base refolding buffers that form a matrix to cover a wide range of strong and weak denaturant conditions for the suppression of protein aggregation. Seven additional buffer additives are used as additional matrix factors, depending on the protein type being refolded. Buffer components can be combined at three concentration levels, allowing a wide spectrum of folding conditions to be tested within one experiment. The adjustable design allows matrix conditions to be tailored to the target protein, preventing sample waste and unneccessary analysis, while maximizing refolding yields.


Delivery information: The kit is accompanied by a Comprehensive Refolding guide with details on isolating, Solubilizing and Purifying inclusion bodies, Optimizing refolding conditions, and analyzing refolding yields.

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