Di-4-ANEPPS membrane stain, for histology

Supplier: Biotium

Synonyms: 4-(2-(6-(Dibutylamino)-2-naphthalenyl)ethenyl)-1-(3-sulfopropyl)pyridinium hydroxide inner salt, 4-(2-(6-(Dibutylamino)-2-naphthalenyl)ethenyl)-1-(3-sulphopropyl)pyridinium hydroxide inner salt

61010
89139-352EA 450.32 CAD
89139-352
Di-4-ANEPPS membrane stain, for histology
Di-4-ANEPPS

Di-4-ANEPPS is a fast-responding membrane potential dye. Changes in the membrane potential of the cell correlate with changes in the fluorescence excitation intensity of the dye.


  • λEx/λEm (MeOH) = 496/705 nm*
  • Orange red solid soluble in DMF, ethanol or DMSO
  • Store at 4°C and protect from light, especially in solution


Di-4-ANEPPS is internalized in cells rapidly, so it is mainly used for short-term experiments. We also offer Di-8-ANEPPS (catalog number 61012), which is more lipophilic and better retained in the outer leaflet of the plasma membrane.


* Note that the spectra of styryl dyes generally undergo a large blue shift in lipids compared to organic solvent. See the spectra for styryl dyes in liposomes under product images.

Formula: C₂₈H₃₆N₂O₃S
MW: 480.66 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD03453609
CAS Number: 90134-00-2

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