Human TOR3A ELISA Kit

Supplier: ANTIBODIES.COM LLC

A312283-96
77212-410EA 877.69 CAD
77212-410
Human TOR3A ELISA Kit
Assays ELISAs

Human TOR3A ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TOR3A in serum, plasma, and other biological fluids.


  • Higher throughput: Get results in just 90 minutes, with a single wash step
  • Detection range: 250 to 4000 ng/L
  • Sensitivity: 9.67 ng/L
  • Sample type: Serum, plasma or other biological fluids
  • Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%


Human TOR3A ELISA kit (A312283) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human TOR3A in An antibody specific for TOR3A has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TOR3A present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-TOR3A antibody, which also binds the TOR3A present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-TOR3A antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-TOR3A antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of TOR3A captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of TOR3A in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.


Ordering information: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, standard solution, standard diluent, biotinylated detection antibody, streptavidin-HRP, wash buffer (25X), substrate solution A, substrate solution B, stop solution, plate sealers, foil pouch, and protocol manual.

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