Human Plasma Kallikrein 1B ELISA Kit

Supplier: ANTIBODIES.COM LLC

A302894-96
76737-026EA 1240.14 CAD
76737-026
Human Plasma Kallikrein 1B ELISA Kit
Assays ELISAs

Human Plasma Kallikrein 1B ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Plasma Kallikrein 1B in serum, plasma, tissue homogenates, and other biological fluids.


  • Ready-to-use ELISA kit
  • Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%


Human Plasma Kallikrein 1B ELISA kit (A302894) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Plasma Kallikrein 1B in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Plasma Kallikrein 1B has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Plasma Kallikrein 1B present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Plasma Kallikrein 1B Antibody, which binds the captured Plasma Kallikrein 1B present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Plasma Kallikrein 1B captured in each well. The concentration of Plasma Kallikrein 1B can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.


Ordering information: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.

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SPECIFICATIONS

Environmentally Preferable
Target protein Plasma Kallikrein 1B
Primary antibody reactivity Human
Assay Type Sandwich
Host
Format Pre-coated
Assay duration Multiple steps
Sensitivity 0.094 ng/ml
Detection range 0.156 - 10 ng/ml
Detection method Colorimetric
Cross reactivity Human Plasma Kallikrein 1B ELISA Kit exhibits high specificity and excellent specificity for the detection of human Plasma Kallikrein 1B. No significant cross-reactivity or interference between Plasma Kallikrein 1B and analogues was observed.
Sample type Serum, plasma, tissue homogenates, and other biological fluids
Sample volume 100 μl
Time to Results 4 h 30 min
Regulatory status RUO
Storage temperature 4 °C
Shelf life Store for 6 months at 4 °C

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