Leukotriene B4 ELISA Kit

Supplier: ANTIBODIES.COM LLC

A303878-96
76734-350EA 1240.14 CAD
76734-350
Leukotriene B4 ELISA Kit
Assays ELISAs

Leukotriene B4 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of Leukotriene B4 in serum, plasma, tissue homogenates, and other biological fluids.


  • Ready-to-use ELISA kit
  • Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%


Leukotriene B4 ELISA kit (A303878) employs the competitive enzyme immunoassay technique for the quantitative measurement of universal Leukotriene B4 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Leukotriene B4 antigen. During the incubation, Leukotriene B4 present in the samples or standards competes with the fixed amount of immobilized Leukotriene B4 for binding sites on the Biotinylated Anti-Leukotriene B4 Antibody. The more Leukotriene B4 present in a sample or standard, the less Biotinylated Anti-Leukotriene B4 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Leukotriene B4 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Leukotriene B4 present in each sample or standard. The concentration of Leukotriene B4 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.


Ordering information: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.

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