Human Leucine Peptidase/LAP ELISA Kit

Supplier: ANTIBODIES.COM LLC

A79057-96
76730-402EA 1006.62 CAD
76730-402
Human Leucine Peptidase/LAP ELISA Kit
Assays ELISAs

Human Leucine Peptidase/LAP ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Leucine Peptidase/LAP in serum, plasma, tissue homogenates, and other biological fluids.


  • Ready To Use ELISA Kit
  • Detection Range: 0.781 to 50 ng/ml
  • Sensitivity: 0.469 ng/ml
  • Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%


Human Leucine Peptidase / LAP ELISA Kit (A79057) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Leucine Peptidase / LAP in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Leucine Peptidase / LAP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Leucine Peptidase / LAP present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Leucine Peptidase / LAP Antibody, which binds the captured Leucine Peptidase / LAP present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Leucine Peptidase / LAP captured in each well. The concentration of Leucine Peptidase / LAP can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.


Ordering information: This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.

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