Human TGF beta 1 ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

KOA0307
76237-192EA 1027 CAD
76237-192
Human TGF beta 1 ELISA Kit, Rockland Immunochemicals
Assays ELISAs

Human TGF beta 1 AccuSignal ELISA Kit


  • Natural and recombinant human TGF beta1.
  • There is no detectable cross-reactivity with other relevant proteins.
  • Expression system for standard: CHO; Immunogen sequence: A279-S390


Transforming growth factor-beta1(TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.


Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human TGF beta 1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of activated human in cell culture supernates, serum, plasma (EDTA) or urine to each empty well. It is recommended that each human TGF beta 1 standard solution and each sample be measured in duplicate.

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SPECIFICATIONS

Environmentally Preferable
Target protein TGF beta 1
Primary antibody reactivity Human
Assay Type Sandwich
Host
Shipping temperature Wet Ice

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