Human TGFBR3 ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

KOA0837
76236-230EA 1150.24 CAD
76236-230
Human TGFBR3 ELISA Kit, Rockland Immunochemicals
Assays ELISAs

Human TGFBR3 AccuSignal ELISA Kit


  • Natural and recombinant human TGFBR3.
  • There is no detectable cross-reactivity with other relevant proteins.
  • Expression system for standard: NSO; Immunogen sequence: G21-D781


Betaglycan also known as Transforming growth factor beta receptor III (TGFBR3), is a cell- surfacechondroitin sulfate / heparan sulfate proteoglycan >300 kDa in molecular weight. Betaglycan binds to various members of the TGF-beta superfamily of ligands via its core protein, and bFGF via its heparan sulfate chains. It is not involved directly in TGF-beta signal transduction but by binding to various member of the TGF-beta superfamily at the cell surface it acts as a reservoir of ligand for TGF-beta receptors. By cDNA array and immunohistochemistry analyses, it is found that TGFBR3 expression is lost in most breast cancers examined in association with loss of heterozygosity of the TGFBR3 locus. TGFBR3 expression decreased during breast cancer progression, and low levels predicted decreased recurrence-free survival in patients. Loss of TGFBR3 through allelic imbalance is a frequent genetic event during breast cancer development that increases metastatic potential. Also, TGFBR3 can localize transformation in the heart and play an essential, nonredundant role in TGF-beta signaling.


Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human TGFBR3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin , EDTA) to each empty well. It is recommended that each human TGFBR3 standard solution and each sample be measured in duplicate.

Order Now

SPECIFICATIONS

Environmentally Preferable
Primary antibody reactivity Human
Host
Shipping temperature Wet Ice

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR