Mouse CD169 - SIGLEC-1 ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

KOA0779
76236-170EA 1704.82 CAD
76236-170
Mouse CD169 - SIGLEC-1 ELISA Kit, Rockland Immunochemicals
Assays ELISAs

Mouse CD169 - SIGLEC-1 AccuSignal ELISA Kit


  • Natural and recombinant mouse CD169.
  • There is no detectable cross-reactivity with other relevant proteins.
  • Expression system for standard: NSO; Immunogen sequence: T20-R1639


SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.


Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse CD169 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD169 standard solution and each sample be measured in duplicate.

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