Human Midkine ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

KOA0727
76235-934EA 1150.24 CAD
76235-934
Human Midkine ELISA Kit, Rockland Immunochemicals
Assays ELISAs

Human Midkine AccuSignal ELISA Kit


  • Natural and recombinant human Midkine.
  • There is no detectable cross-reactivity with other relevant proteins.
  • Expression system for standard: E.coli; Immunogen sequence: K23-D143


Midkine(MK or MDK) also known as neurite growth-promoting factor 2(NEGF2) is a protein that in humans is encoded by the MDK gene. Midkine is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin(NEGF1, 46% homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. MK is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. A molecular complex containing receptor-type tyrosine phosphatase zeta(PTPζ), low density lipoprotein receptor-related protein(LRP1), anaplastic leukemia kinase(ALK) and syndecans is considered to be its receptor.


Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml, 78pg/ml human Midkine standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Midkine standard solution and each sample be measured in duplicate.

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