Human TGF-beta 3 ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

KOA0628
76235-778EA 1150.24 CAD
76235-778
Human TGF-beta 3 ELISA Kit, Rockland Immunochemicals
Assays ELISAs

Human TGF-beta 3 AccuSignal ELISA Kit


  • Natural and recombinant human TGF-beta 3.
  • There is no detectable cross-reactivity with other relevant proteins.
  • Expression system for standard: sf21; Immunogen sequence: A301-S412


Transforming growth factor beta 3(TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix(ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin(CTNNB1) via nuclear phospho-Smad2 and Smad4.


Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human TGF-beta 3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of activated human cell culture supernates, serum, plasma(EDTA) or urine to each empty well. It is recommended that each human TGF-beta 3 standard solution and each sample be measured in duplicate.

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SPECIFICATIONS

Environmentally Preferable
Primary antibody reactivity Human
Host
Shipping temperature Wet Ice

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