EnzyChrom™ Fatty acid Assay Kit, BioAssay Systems

Supplier: BIOASSAY SYSTEMS

EFFA-100
75878-084EA 1425.1 CAD
75878-084
EnzyChrom™ Fatty acid Assay Kit, BioAssay Systems
Assays Cellular Assays

For quantitative determination of fatty acid and evaluation of drug effects on its metabolism.


  • Linear detection range colorimetric assay 7 - 1000 μM, fluorimetric assay 7 - 100 μM fatty acid
  • Convenient "mix-and-read" high-throughput assay


Fatty acids are aliphatic monocarboxylic acids that are ubiquitously found in animal or vegetable fat, oil and wax


BioAssay Systems’ method provides a simple, one-step and high-throughput assay for measuring free fatty acids. In this assay, free fatty acids are enzymatically converted to acyl-CoA and subsequently to H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the free fatty acid concentration in the sample.


Fatty Acids play important roles in cellular synthesis, energy metabolism and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome and Reye Syndrome.

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SPECIFICATIONS

Environmentally Preferable
Method OD570 nm or FL530/585 nm
Processing time 30 minutes
Sample Serum, plasma, urine, saliva, milk, cell cultures, food, agriculture etc.

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